A river runs through it …

Loire_at_Orleans_Sept19

I made an journey via Paris to Orleans last weekend to wave off my wife and daughter as they walk part of the Camino de Santiago.   The part of this austere Medieval pilgrimage route that they chose to follow happens to be one that meanders along the Loire Valley, passing chateaux and wineries en route.   Apparently, blisters are providing an element of self-mortification to keep the spirit of pious ascetism alive.

Orleans has a beautiful Medieval old town, dominated by a cathedral, along with plenty of reminders that it was here that Joan of Arc whupped the English.   More importantly, for a freshwater ecologist, a river runs through it.   The mighty Loire – the longest river in France – rises in the Masif Central and then flows north until it reaches Orleans, then it swings round to flow west to join the Atlantic Ocean at Nantes, a total length of just over 1000 km.  The river at Orleans is broad and shallow, divided into two channels by a wooded island; the left-hand chnnel is braided, with many small gravel islands, some with grass and herbaceous vegetation, and the water is generally shallow.  However, the bed of the river itself was a dark green in colour.  The Loire Valley is known as the “Garden of France” and this gives a clue to the scale of nutrient enrichment that we might expect in the river.  In the backwaters, this green backdrop was enlivened by patches of red due to the aquatic fern Azolla (see “Escape to Southwold”).

Azolla_Loire_Sept19

Patches of Azolla floating over algae-smothered substrata in a backwater of the River Loire at Orleans.  The picture at the top of the post shows a view looking downstream from the left side of the Pont George V. 

I brought a sample of the algae from some stones that were just within reach of the shore home on the Eurostar in a Perrier Water bottle for a closer inspection and was surprised by the diversity. In particular, I noticed several clumps of a narrow cyanobacterium that proved hard to photograph (possibly Homoeothrix janthina: see “Algae from the Alto Duoro”) along with many green algae.  I also saw Cladophoraglomerata, which is one of the classic indicators of nutrient rich conditions, along with Stigeoclonium, two forms of Spirogyra, two forms of Oedogonium and myriad green unicells and coenobia.  Stigeocloniumis another good indicator of nutrient enrichment,as  the filaments narrow to long colourless “hairs” when key nutrients are scarce – these cells are physiological adaptions to scavenge phosphorus and their absence at Orleans shows that this nutrient is not in short supply (see “A day out in Weardale”).

Stigeoclonium_Loire_Orleans_Sept19

Stigeoclonium cf. tenue (based on properties of erect filaments) from the River Loire at Orleans, September 2019.  Scale bar: 10 micrometres (1/100thof a millimetre). 

As well as green algae and cyanobacteria, there were also a lot of diatoms.  A few of these are illustrated below, and I’ll describe the diatoms in more detail in a future post.   As well as those I have photographed, I also saw long chains of a species of Fragilaria and another small araphid (possibly Staurosira) as well as Ulnaria ulna and some cells of Gomphonema and Navicula.   Note, in particular, the tube of Encyonema (possibly E. prostratum).   I’ve talked about tube-forming diatoms before (see “An excuse for a crab sandwich, really …”) but still can’t really explain what advantage this structure confers on a diatom.   What we can, perhaps, say, is that tube-dwelling is one of a several habits represented in the diatoms from Orleans – chains, erect, free-living motile, epiphytes  and more – and the mass of other algae create a rich diversity of microhabitats that the diatoms can exploit.

diatoms_Loire_Orleans

Some diatoms from the River Loire at Orleans: a. – d. Diatoma vulgare; e. Cocconeis pediculus; f. Encyonemasp.   Scale bar: 10 micrometres (= 1/100thof a millimetre).  The identity of the tube-dwelling form will have to wait until a cleaned sample is available. 

This abundance and diversity of green algae reminded me of some of the lush growths I had seen in UK rivers during the 2018 heatwave (see “Talking about the weather …”).  In a healthy river in the UK, I would expect to find less conspicuous growths than this, as invertebrate grazers would keep the algal biofilm shorn to a short stubble.   If, for any reason, the invertebrates cannot keep up with the algal growth, then a feedback loop is set up: the algae suck the valuable oxygen the invertebrates need from the water, the grazing reduces in intensity yet further, leading to a situation such as that I saw in the River Loire is the result.

Warm weather exacerbates the situation because water holds less oxygen at high temperatures.  In my posts about the River Wear last summer I commented that the plants in the river suggested that the river was more enriched with nutrients than was actually the case and I suspect that this was the result of these interactions.   The more southerly, more continental climate of the Loire Valley will experience these effects more often and it is possible that what I am looking at in Orleans may become the norm for UK rivers, as global warming intensifies.  Over the past decade I’ve worked on how to estimate the threshold concentrations of nutrients that a healthy river can endure.  However, nutrients rarely exert a direct effect on the plants and animals in a river but, instead, work through their effect on other factors such as oxygen. That will mean that global warming may wipe out any benefits of expensive nutrient reduction programs based on current estimates of the relationship between nutrients and river ecosystem health.  That’s a rather depressing prospect.

So I’ll end on a more cheerful note: the reason Heather and Rosie chose to start their Camino in Orleans was that they wanted to avoid a flight if possible.  At least that is how they sold it to me.   That they also chose to start their pilgrimage in a town close to the Sancerre vineyards may be pure coincidence.   Or maybe not ….

References

Surt, M.M., Jansen, M.A.K., Harrison, S.S.C. (2011).  Invertebrate grazing and riparian shade as controllers of nuisance algae in a eutrophic river. Freshwater Biology 56: :2580-2593

Wilco, C.E., Verbrak, P., Durance, I., Vaughn, I.P. & Ormerod, S.J. (2016).  Field and laboratory studies reveal interacting effects of stream oxygenation and warming on aquatic ectotherms.  Global Change Biology 22: 1769-1778.

Orleans_cathedral_Aug19

Orleans cathedral, August 2019.

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Follow the data, stupid …

A perennial problem with ecology is that it is a discipline that is far better at describing problems than it is at solving them. The Water Framework Directive (WFD) encapsulates this: after nineteen years, we have a pretty good idea of the condition of Europe’s waters but have made very little progress in restoring the half that do not yet achieve good ecological status.

The reason for this is, I suspect, because describing the problem is a task that lies squarely within the remit of a scientist whilst finding solutions requires interactions that go beyond the boundaries of science, encountering vested interests along the way.   The agricultural sector’s enthusiasm for the environment is tempered by their desire to maximise yield and earn a living from the land, politicians are wary of regulations that may deter business or raise prices for the consumer and all of us are too wedded to the luxuries that the modern world offers.

The WFD can be seen as an embodiment of the social contract, articulated by philosophers such as Thomas Hobbes whereby individuals forego some rights in order to transcend the state of nature (“… nasty, brutish and short.”) and give us access to the benefits of an ordered society.  In this case, we all consent to forego some freedoms in return for a share in the benefits that a healthy aquatic environment will bring to all of us.   “Freedom” might seem like a weighty word in this context but anyone who has watched their sewerage charges creep steadily upwards over the past twenty years should recognise this as the price we pay for the freedom to flush away life’s less desirable by-products.

The problem is defining the point at which we hand over our natural rights to a higher authority.   We understand this when driving: an urban speed limit of 30 miles per hour reflects the point at which the risk we pose to other road users are deemed societally unacceptable and our right to drive as fast as we wish has to be curtailed.  If we can translate that principle into environmental governance then we can set “speed limits” for the major pressures that impact on the aquatic environment.   How do we get from an ecologist’s understanding of a “healthy” river (“good ecological status”, in WFD parlance) to the “speed limit” for nutrients, widely recognised as one of the major pressures affecting both freshwater and marine systems?

That’s been the focus of some work I’ve been doing under the auspices of the European Commission’s Joint Research Centre, one strand of which has just been published in Science of the Total Environment.  This paper looked at the threshold concentrations for nutrients (phosphorus and nitrogen) used by EU countries, noting the very wide range of values chosen as the national “speed limit”.   The situation is complicated because, just as is the case for roads, different types of rivers require different limits and we had to look for variation between countries amidst an array of variation within countries.   What emerged, however, was a clear relationship between the threshold values and the method used to set the standard.  Those that had applied statistical or modelling techniques to national data generally had tighter thresholds than those that relied upon “expert judgement”.  I’ve included the two figures from this paper that make this point.

Poikane_et_al_2019_Fig7

Range of good/moderate lake phosphorus (a) and nitrogen (b) threshold values grouped by method used to determine the value. Different letters indicate groups that are statistically different (p ≤ 0.05).   Fig. 7 from Poikane et al. (2019).

“Expert judgement” is one of those slippery terms that often creeps into official reports.   There needs to be space within a decision-making process for an experienced professional to see through the limitations of available evidence and present a reasoned alternative.  However, “expert judgement” too often becomes a shorthand for cutting corners and, in this case, grabbing numbers from the published literature that seem vaguely plausible.  There is also a darker side because, having unhitched decision-making from the evidence, “expert judgement” can become a euphemism for the “art of the possible”.  I have seen this occur during discussions around setting and revising river phosphorus standards in the UK: the regulators themselves are under pressure to balance environmental protection with economic development and tight standards can potentially limit what can be done in a catchment.

Another of our recent papers (this one’s not open-access, I’m afraid) shows that setting standards using empirical models is far from straightforward and we also recognise that standard setting is just one part of a longer process of nutrient management.   However, setting inappropriate standards simply as an expedience seems completely barmy, as you are never going to attain your desired ecological benefits.   The cynical view might be that, as the process of environmental change is invariably greater than the electoral cycle, there is limited accountability associated with such decisions, compared with more immediate political capital kudos from bringing investment and jobs to a region.

Poikane_et_al_2019_Fig8

Range of good/moderate river phosphorus (a) and nitrogen (b) threshold values grouped method used to determine the value. Different letters indicate groups that are statistically different (p ≤ 0.05).   Fig. 8 from Poikane et al. (2019).

All of our work has shown that, in most cases, the relationship between biology and nutrients is weak and, for this reason, large datasets are needed if robust inferences are to be drawn.  This leads to one further consequence of our work: setting environmental standards may only be possible if countries pool their data in order to produce big enough datasets with which to work.  This is particularly the case for smaller countries within the EU, but also applies to water body types that may be relatively infrequent in one country but are more widespread elsewhere.   I had recent experience of this when working on the Romanian stretches of the Danube: they simply did not have a wide enough gradient of conditions in their own territory, and we had to incorporate their data into a larger dataset in order to see the big picture (see “Beyond the Tower of Babel …”).    Writing about the benefits of international collaboration as the Brexit deadline looms obviously has a certain irony, but it needs to be said.  Far from being the distant and unaccountable law maker of Brexiteer mythology, in this field the European Commission has been quietly encouraging Member States to share experience and promote best practice.  One can only speculate about the future of the UK environment once free of Brussels oversight.

References

Philips, G., Teixeira, H., Poikane, S., Salas, F. & Kelly, M.G. (2019).   Establishing nutrient thresholds in the face of uncertainty and multiple stressors: a comparison of approaches using simulated data sets.   Science of the Total Environment684: 425-433.

Poikane, S., Kelly, M.G., Salas Herrero, F., Pitt, J.-A., Jarvie, H.P., Claussen, U., Leujak, W., Solheim, A.L., Teixera, H. & Phillips, G. (2019).  Nutrient criteria for surface waters under the European Water Framework Directive: Current state-of-the-art, challenges and future outlook.  Science of the Total Environment 695.  

Note on figures:

The methods used by Member States to derive nutrient thresholds are described in more detail in Poikane et al. (2019).   In brief, the approaches are:

1 – regression between nutrient and biological response;

2 – modelling;

3 – distribution of nutrient concentrations in water bodies classified (using ecological criteria) as high, good or moderate status;

4 – distribution of nutrient concentrations in all water bodies using an arbitrary percentile;

5 – expert judgement.  This includes values taken from the literature or from older European Directives. For example, for nitrate, the common use of the value 5.65 mg-N L−1 in freshwaters is likely to be derived from the guideline value of 25 mg L−1 of nitrate in the Nitrates Directive (91/676/EEC) or now repealed Drinking Water Directive (80/778/EC).

6 – The so-called OSPAR Comprehensive Procedure is used widely in coastal and transitional waters. In this, a water body is considered to be an ‘Eutrophication Problem Area’ if actual status deviates 50% or more from reference conditions.

7 – insufficient information.

Tales from prehistory

Stonehenge_Aug19

Microscopes and Monsters has been quiet for a couple of weeks, as I have been on holiday, part of which was spent “off grid” at the Green Man Festival in Wales.  From there, we headed to London for a Proms concert (two music festivals in a week!) via the Cotswolds and the ancient landscapes of Salisbury Plain.

My first visit to Stonehenge was 50 years ago, at which time you could pull off the A303 and wander amongst the columns unconstrained by fences and barriers.   Now the visitors are guided to a visitor centre two kilometres from the site, and offered a shuttle bus after being relieved of £20.  Or, if you prefer, you can walk across Salisbury Plain to the monument.  On a sunny afternoon in August this becomes part of the experience as there are ancient burial mounds (some pre-dating Stonehenge) both alongside the path and dotted around the horizon.   Stonehenge itself gradually rose up ahead of us, and we experienced a little of what the ancients must have felt as they approached Stonehenge along the processional way.

The last time that I was here was a stop off between field work on the nearby River Wylye and a meeting in Reading.   At the time I was engaged with two separate projects concerned with the health of chalk streams, which are characteristic of this part of southern England.   The approach we used elsewhere in the country was to compare what we found in samples we collected with what we expected to find if that site was in a pristine condition.

There was, at the time, a vigorous debate about how this “reference condition” should be defined.   This debate had a theoretical component (epitomised by Brian Moss’ paper in the reference list) but also a more pragmatic element (encapsulated by the other paper).  This was necessary because an ultra-strict, but theoretically sound, approach might not yield enough data from which a robust prediction of the “expected” ecology could be derived.   In essence, we searched out remote regions of the UK where population density was low and agriculture was not intensive and used these to derive our understanding of what to expect in the more densely-populated regions of the country.

Avebury_Aug19

Part of Avebury stone circle, Wiltshire, August 2019.  The photograph at the top of the post shows Stonehenge.

This worked quite well (although Brian Moss, predictably, had his own pithy thoughts on the approach).  However,  we simply could not find any sites that fulfilled our criteria of low population density and a low intensity of farming in those parts of lowland Britain where the underlying geology was Jurassic limestone, Cretaceous chalk or another formation that resulted in very hard water.  Our estimates of ecological health in such regions depend, as a result, on extrapolation and judgement rather than evidence.   That is all well and good for an academic journal but, in the case of the River Wylye, Wessex Water were being asked to spend hundreds of thousands of pounds to upgrade sewage works and, rightly, felt that they needed something more in order to explain the consequent price rises to their customers.

The OS maps of the region around my sampling points on the Wylye were dotted with symbols marking ancient monuments (long and round barrows, in particular) and the huge, mysterious religious sites of Stonehenge and Avebury lie just to the east.  Together, they point to continuous occupation of the area for over four thousand years, which means that it is hardly a surprise that we found no sites that met our criteria for a “pristine” stream.   The chalk streams of southern England are famous and rightly regarded as a threatened habitat, but they are not natural.  It is better to think of them as aquatic equivalents to hay meadows or hedgerows: ecologically-rich habitats that have been created by human activity, rather than as a result of “natural” ecological processes.

That means that it we need to diverge from a strict definition of “reference conditions” in order to set a baseline for ecological expectations in such circumstances.  For macrophytes – the larger aquatic plants – there is an expectation that the flora in this baseline state will be rich; however,  this assumption does not work for the microscopic algae in chalk streams.  We also found that river stretches where the macrophytes are thriving and, apparently, healthy, often have diatoms that suggest nutrient enrichment.  That is a puzzle for which we think we may have a solution, and which I will write about in a future post.

Silbury_Hill_Aug19

Silbury Hill, part of the Avebury World Heritage Site.  It is a Neolithic site whose original purpose is unknown though, to a visitor from north-east England, it looks remarkably like a slag heap.

We use low population density and absence of intensive agriculture as a proxy for “natural” in the uplands but need to treat this assumption with care too.  There might be fewer grand Neolithic monuments in the north of England or Scotland but signs of ancient habitation are there if you care to look (see “More reflections from the dawn of time”).   The moorland where these streams rise is, itself, an artificial habitat, created when early agriculturalists removed the natural tree cover.  Modern streams in these areas are, therefore, exposed to more light than in their primeval states and that will have important consequences for the plant life that lives within them.  They may be the best we have, but are hardly “natural”.

Two factors, both highly pragmatic, brought this debate to a close.   The first was realisation that, whatever the rights and wrongs of purist versus practical standpoints, most of our rivers are very degraded and alterations in the approach used to define the “expected” condition would be unlikely to change this broad scale picture.   About sixty-five per cent of our rivers fail to achieve good ecological status despite the flaws in the reference concept.  The second factor was simply that, since the financial crisis in the 2008-2010, the UK environment agencies have had too few resources to improve the reference concept.   As any such “improvement” will almost certainly make the true state of UK rivers look even worse than it does at the moment, a more cynical argument is that few of the bureaucrats involved in the process have any great enthusiasm for the task anyway.

References

Moss, B. (2008).  The Water Framework Directive: total environment or political compromise.  Science of the Total Environment400: 32-41.

Pardo, I., Gómez-Rodríguez, C., Wasson, J.G., Owen, R., van de Bund, W., Kelly, M., Bennett, C., Birk, S., Buffagni, A., Erba, S., Mengin, N., Murray-Bligh, J. & Ofenböeck, G. (2012).  The European reference condition concept: A scientific and technical approach to identify minimally-impacted river ecosystems.  Science of the Total Environment420: 33-42.

 

The presence of absence in Castle Eden Dene

CED_Aug19

Some of my strongest impressions of Castle Eden Burn after last week’s visit concerned not what I found in the stream, but what was not there.  I mentioned in my previous post that I had not seen the mosses that I associated with streams in northern England in Castle Eden Burn, but there were other species, too, that I had expected to see but had not noticed.   Once I have noticed that something is absent, this absence becomes present.  I have noticed the presence of absence.  Woohoo: I’ve shoehorned Jean-Paul Sartre’s Being and Nothingness into a blog about ecology.

When I got back home I had read a chapter about the FBA’s study of the Winterbourne in Dorset, an intermittent stream flowing off the chalk downland, and noticed that they had recorded plants there that I knew from north-east English rivers, but which I could not remember seeing in Castle Eden Burn.  Was this because I had not searched the stream environs thoroughly, or is this a real difference between intermittent streams on chalk and on Magnesian limestone?

I went back this weekend to try to answer these questions, taking Heather with me, as her skills with the higher plants far exceed mine, and walked as much of the stream bed as we could, starting near the remains of a footbridge at NZ 424 389, and making our way downstream to Denemouth, where Castle Eden Burn joins the North Sea.  If my original intention was to better understand the burn by traversing space within the Dene, my first lesson concerned time: a week with some heavy rainfall separated my two visits and it was clear straight away that the Burn had been flowing during the week, with a fine layer of silt and mud spread across much of the surface, making parts of it slippery to walk upon.  There were standing pools of water at several points in the upper part of the Burn too.   Within a week the stream had come and gone, offering scant opportunities for any water-loving organism to establish.

We made our way along the Burn through the delicious silence of the forest.  The banksides were richly vegetated: masses of opposite-leaved golden saxifrage plus the mosses I described last time and many others, along with plenty of harts-tongue fern (Asplenium solopendrium).   Then, with a very clear demarcation, there was the stony stream bed with very little vegetation at all.    We looked hard for three plants, in particular, that I associated with the damp margins of streams, and which I had expected to see here: Verronica beccabunga (brooklime or water speedwell), Rorippa nasturtium-aquaticum (water cress) and Mentha aquatica (water mint).  None seemed to be present in any of the stretches we visited apart from a single sorry looking brooklime in the freshwater marsh at Denemouth .

What we did find, a little further downstream, was a pebble and gravel-dominated stretch with a straggly array of plants, all bent over in the direction of flow.   These included broad-leaved dock (Rumex obtusifolius), nettles (Urtica diocia), a few shoots of Himalayan balsam (Impatiens glandulifera) and some grasses.    Were I not standing on a dry stream bed I would have assumed that this was a bare piece of ground being colonised by typical ruderal species.  And that, I think, offers some insights into the ecology of Castle Eden Burn.   This is not a stream that occasionally dries out: it is a long-thin terrestrial habitat that is occasionally flushed through by water.   Welcome to north-east England’s premier wadi.

Rumex_obtusifolius_CED

Rumex obtusifolius and other ruderal vegetation on the stream bed of Castle Eden Burn, August 2019.

This hypothesis really needs corroboration by a hydrologist, but the graph I showed in “Out of my depth …” shows that, despite flow being generally low,  episodes of high flow are scattered throughout the year, and I suspect that these keep the substratum mobile and, more important, stop organic matter accumulating to give amphibious plants an opportunity to establish.   The water table, too, I guess, is too far below the stream bed in between the spates to make it easy for plants to stay hydrated.   This is one of the main differences between Castle Eden Burn and the southern chalk streams, which are characterised by very stable flow regimes

From the point at which Blunt’s Burn enters Castle Eden Burn (NZ 436 396) there does seem to be permanent flow down to the sea.  Still, however, there was very little in-stream vegetation.  That was in contrast to the forest around us, which was floristically-rich (Heather has written more about this on her blog) and, on this warm summer morning, positively humming with bees and aflutter with butterflies.

A large embankment takes the busy A1086 over the Dene, the Burn passing through a long culvert at this point, after which there is a viaduct taking the coastal railway line across before the dene widens out into a large area of meadow just before it reaches the sea.   The stream’s path to the sea is, however, blocked by mine waste that was dumped from the coal mines that used to line the Durham coast.  This forces the stream to turn ninety degrees south for a few hundred metres before finding a way through and, gradually, trickling and percolating through the beach. The mines have all gone now and the sea is gradually eroding this compacted mass of waste.  Before the waste arrived, apparently, there was an area of saltmarsh at the mouth of the burn.   Now, there is a freshwater marsh, dominated by reeds (Phragmites australis).  When the mine waste finally goes, maybe the saltmarsh will return.  Meanwhile, Castle Eden Burn has no grand finale: it ends on a whimper not a bang.

We climbed a narrow, steep pathway up through gorse and brambles onto the clifftops overlooking these final stages of Castle Eden Burn to get a view that was, in light of all that had passed through my mind earlier, oddly symbolic.  The stream flowed almost due east until it encountered the bar, and the gentle arc which it then describes looks just like a question mark.   How ironic, I thought, for a stream that raises more questions than answers to sign off in that way ….

Denemouth_CED_Aug19

Denemouth, at the end of Castle Eden Dene, just above the point where the stream joins the North Sea.

A thousand little mosses …

Castle_Eden_Burn_July19

Nature doth thus kindly heal every wound. By the mediation of a thousand little mosses and fungi, the most unsightly objects become radiant of beauty. There seem to be two sides of this world, presented us at different times, as we see things in growth or dissolution, in life or death. And seen with the eye of the poet, as God sees them, all things are alive and beautiful.
Henry David Thoreau (journal entry, March 13, 1842)

I was back in Castle Eden Dene earlier this week for my regular visit and, once again, encountered a dry stream bed.  This was no great surprise but, having written about the algae of dry river beds in earlier posts from Castle Eden Dene (see “When the going gets tough“ for the most recent instalment), I thought that I would focus on some of the other vegetation that I could see in and around the stream and, in particular, the bryophytes.   I asked Gaynor Mitchell, who wrote her MSc thesis on the bryophytes of the Dene, to come along and help me with these as my skills never really extended beyond those mosses and liverworts that live permanently submerged in streams and, as we have seen, there is rarely enough water in the burn here for such species to thrive.

There is a rich carpet of mosses on the woodland floor in much of Castle Eden Dene but, in the stream bed and its immediate environs, it is thalloid liverworts that are the most conspicuous bryophytes. Two species, in particular, stand out: the first is Conocephalum conicum, which has broad ribbon-shaped branches and an upper surface covered with pores – which just visible as light coloured dots to the naked eye.   The other is Pellia epiphylla, which was particularly noticeable on the top surface of boulders that are, I suspect, rarely covered, even when the burn is very full.   P. epiphylla had smaller thalli than C. conicum and, importantly, lacked the distinct pores on the upper surface.

Conocephalum_conicum_CED

Conocephalum conicum from Castle Eden Dene, July 2019. The pores are clearly visible on the thallus in the lower image.

Pellia_epiphylla_CED

Pellia epiphylla from the top of a boulder in Castle Eden Burn, July 2019

Alongside Pellia epiphylla on the boulder tops were shoots of the moss Thamnobryum alopercum.  The populations on top of the stones were rather non-descript to the naked eye, being stems growing horizontally across the rock surface. However, amidst these, we found a few of the upright stems which have a distinctly tree-like appearance.   We found more characteristic growths on the woodland floor nearby and my now-dated copy of Watson does, in fact, comment that this species has these two distinct habitats and also that it is a good indicator of calcareous conditions (for anyone who had not noticed the towering limestone cliffs in Castle Eden Dene, I presume?).   Lower down (and, thus, more frequently submerged), we saw Rhynchostegium confertum though this, too, is a species more often associated with terrestrial rather than aquatic habitats.  More significantly, the mosses I associate with streams in north-east England – Rhynchostegium riparioides, Fontinalis antipyretica and Leptodictyon riparium – are all missing from Castle Eden Burn.

Thamnobryum_allopercum_CED

Tree-like shoots of Thamnobryum alopercum from the forest floor in Castle Eden Dene in July 2019.  Growths on rocks in Castle Eden Burn were smaller but there were enough upright stems for it to be recognisable with the naked eye. 

Gaynor’s sharp eye spotted many other mosses and liverworts, though more in the woodland around the stream than in the stream bed itself.  As well as mosses and liverworts, the stream’s vegetation also consisted of a number of grasses and patches of Chrysoplenium alterniflorum, opposite-leaved golden saxifrage.

The story that the vegetation is telling is, I would venture, that Castle Eden Burn is a shaded terrestrial habitat that is occasionally wet, rather than an aquatic habitat that is often dry.  I dug out an old account of the Winterbourne Stream, an intermittent stream in the chalk downlands of southern England for comparison, and found little overlap in the species recorded.   Care is needed for this comparison as the focus of the surveys is different (the Winterbourne account, for example, includes no bryophytes and spans perennial as well as intermittent sections) but there was a mix of genuinely aquatic and amphibious species, including Callitriche sp. and aquatic Ranunculus, which I did not see in Castle Eden Burn.    I suspect that Castle Eden Burn spends longer as a dry stream bed than the upper parts of the Winterbourne.  However, we also must remember that the Winterbourne data are now almost 50 years old, so that stream, too, may have changed much in the interim.

All this adds to my opinion that Castle Eden Burn – and the streams flowing through the other coastal denes in County Durham – are a unique and understudied habitat.  And that’s before I start thinking about the animal life here…

Chrysoplenium_alterniflorum_CED

A patch of Chrysoplenium alterniflorum, opposite-leaved golden saxifrage, on the bed of Castle Eden Burn, July 2019.

Reference

Berrie, A.D. & Wright, J.F. (1984).  The Winterbourne Stream.   pp.179-206.  In: Ecology of European Rivers (edited by B.A. Whitton).  Blackwell, Oxford.

Mitchell, G. (2015).  Bryophytes: changes in diversity and habitat in Castle Eden Dene (1975-2011).   Northumbrian Naturalist: Transactions of the Natural History Society of Northumbria 79: 39-66.

Watson, E.V. (1981).  British Mosses and Liverworts. Third Edition.  Cambridge University Press, Cambridge.

Hilda Canter-Lund competition winners 2019

Zoe_Loffler_Synphony_of_Seaweed

This year’s Hilda Canter-Lund competition for the best algal-themed photograph has been won by Zoe Loffler for her image “Symphony of Seaweeds” taken on a at low tide near Apollo Bay, Victoria, Australia, while on a family camping trip.  She took the photo using a Google Nexus 5x Smartphone.  Zoe grew up diving in temperate waters near Melbourne, Australia. She completed her undergraduate degree and PhD at James Cook University in Townsville, Australia, studying the ecology of canopy-forming seaweeds (particularly Sargassum spp.) on coral reefs. She is now based in Sydney, and enjoys snorkelling and diving in temperate waters where there is such a wonderful diversity and abundance of seaweeds. The image meets Henri Cartier-Bresson’s maxim of the “decisive moment” (see “How to win the Hilda Canter-Lund prize”) and Zoe comments in her caption that the photo shows all who are unfamiliar with seaweeds that “they are not just brown and smelly!”.

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Zoe Loffler: winner of the 2018 Hilda Canter-Lund prize for algal photography, for her image “Symphony of Seaweed”, shown at the top of the post.

Since 2016 we have also offered a second prize which is awarded to a photograph in a contrasting style to the overall winner.  This year, that prize goes to Damien Sirjacobs of the University of Liege in Belgium  for his image “Blue Haze”. This shows a bloom of benthic blue diatoms of the genus Haslea (H. ostrearia, H. provincialis) covering a community of macroalgae (Padina pavonica, Acetabularia acetabulum, Halopteris scoparia, Dictyota sp.) in the shallow water of Calvi Bay (Corsica, France). For scale, the circular caps on the end of the Acetabularia stalks are 5 – 10 mm in diameter.   The image was taken at a depth of four metres in May 2018 with a LUMIX TZ10 in an underwater housing, under natural light conditions, while scuba-diving along rocky shores of the Revellatta peninsula (Calvi Bay).

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Damien Sirjacobs’ image: Blue haze”.  

There is a lot to interest readers of this blog in Damiens’s image; first of all, Acetabularia is another challenge to the generally-accepted view that multicellularity is the only option for large organisms.  Although the plant is quite large and, unlike Vaucheria is more elaborate than a simple tube of cytoplasm (see “The pros and cons of cell walls …”) .  The whole organism is, in fact, just one giant cell with a single nucleus.  

The diatom Haslea that grows over Acetabularia and the other macroalgae in Damien’s picture creates a blue haze due to a pigment called “marennine” which is found in vacuoles inside the cells (as you can see in the photograph below).   When marennine-containing species of Haslea are abundant around oyster beds (as is the case in parts of Brittany), then the pigment turns the gills of the oyster green and such oysters are highly sought after by gastronomes.   Whether or not these oysters really taste better is debatable but marennine certainly does have some antimicrobial properties.

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Damien Sirjacobs: co-winner of the 2019 Hilda Canter-Lund prize for algal photography.

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Whilst Zoe’s image has direct visual appeal, and most people will recognise it almost straightaway as depicting seaweeds, Damien’s image has a more other-worldly quality.   Unless you are familiar with the habitats and organisms, then it is difficult to interpret what is portrayed (see “Abstracting from reality …”).   One of the challenges of photographing algae is that we are dealing with the real yet little understood aspects of biodiversity, creating a multi-layered problem: first, of capturing an impression of the organism(s) but, also,  of interpreting the image to a lay-audience.   In the case of Blue Haze we have that intriguing combination of beauty, mystery and economic relevance.   That is what makes phycology such a fascinating subject.

Reference

Gastineau, R., Prasetiya, F.S., Falaise, C., Cognie, B., Decottignies, P.,  Morançais, M., Méléder, V., Davidovich, N., Turcotte, F., Tremblay, R., Pasetto, P., Dittmer, J., Bardeau, J.-F., Pouvreau, J.-B. & Mouget, J.-B. (2018). Marennine-like pigments: blue diatom or green oyster cult?   pp. 529-551.  In: Blue Biotechnology: Production and Use of Marine Molecules (edited by Stéphane La Barre and Stephen S. Bates).  Wiley VCH Verlag GmbH & Co. KGaA

The intricate life of a colonial alga …

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The annual Algal Training Course in Durham always has a field trip out to Cassop Pond, a small pond at the foot of the Permian Limestone escarpment in County Durham that has featured in a few of my posts over the years (see “A return to Cassop”).  This year, the group came back with some samples from the pond’s margins bearing a suspension of green dots just visible to the naked eye which, when examined under the microscope, turned out to be the colonial green alga Volvox aureus.  These are spherical, with the cells at the periphery, joined together by thin strands of protoplasm. The smaller colonies were scooting about, propelled by the pairs of flagellae borne by each of the cells that constitute the colony, whilst the larger ones (mostly “pregnant” with one or more daughter colonies) were sessile.

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Volvox aureus colonies just visible to the naked eye in a drop of water from Cassop Pond, July 2019.   The drop is 13 millimetres across.

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Colonies of Volvox aureus (each bearing daughter colonies) from Cassop Pond, July 2019.  Scale bar: 50 micrometres (= 1/20thof a millimetre).

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A close-up of part of a colony of Volvox aureus from Cassop Pond, July 2019.  Scale bar: 20 micrometres (= 1/50thof a millimetre). 

Watching a Volvox colony swimming around under the microscope is a beguiling experience, but its movement is not random.  Consider: there may be a 1000 or more cells in the larger colonies, each with two flagellae.  If all beat their flagellae at random, the colony would not get anywhere, as the force in one direction would be cancelled out by forces in all other directions.   But Volvox colonies do actually move with intent.   Look closely at the individual cells in the photos below and you will see that each has a red-coloured eye spot (the light-detecting organelle actually lies beneath the red layer, which acts as a filter).   People with more patience than me have noticed that the eye spots in different parts of the colony differ in size, suggesting a level of organisation that may not be immediately apparent.  We also know that the daughter colonies tend to form at the posterior end of the colony (assuming “posterior” and “anterior” in a spherical colony are defined by the direction of travel) and also that only a small number of cells (larger than the others) are responsible for the division that produces these.

In theory, a spherical object is going to offer less resistance and so sink faster than an object of the same size that had a greater surface area : volume ratio. This should mean that they are not able to stay in the light-rich surface layers where they can photosynthesise and grow.   In practice, Volvox colonies are able to adjust their position by using their flagella but this requires them to pump some of the energy they have obtained from photosynthesis into the flagella’s motors.  Another advantage in Volvox’s favour is a relatively low density of the colony as a whole.  The individual cells are separated by strands of protoplasm which creates a lattice through which water can penetrate, so the overall density of the colony is closer to that of the surrounding water than would be the case if the cells were tightly packed.

Volvox is most often found in the summer in relatively nutrient rich lakes, where nutrients are sufficiently plentiful to support a rich crop of algae.  A motile colony that is not too dense is well-placed to adjust its position to stay in the surface layers and harvest the sunlight.  Moreover, the size of the colony probably means that it is too big for the filter-feeding zooplankton that grazes on the algae.   At the same time, however, Volvox begins to experience some of the problems associated with multicellular life (see references in “The pros and cons of cell walls …”).   As large multicellular organisms ourselves, a nuanced discussion about the pros and cons of multicellularity may seem to only have one possible outcome.   However, Volvox inhabits a world where plenty of single-celled organisms thrive and where a colonial lifestyle offers a small competitive advantage.  It means that it is quite happy drifting around at the time of year when many of us would like nothing better than to don swimming trunks and soak up some sun in a local pool.   Study algae for too long and you end up realising that only losers need to evolve.

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Cells from a Volvox aureus colony from Cassop Pond, July 2019. You can see the red eye-spots in some of the cells in the left-hand image (bright-field) whilst the protoplasmic strands joining cells together can be seen in the right-hand image (phase contrast).   Scale bar: 10 micrometres (= 1/100thof a millimetre). 

References

Canter-Lund, H. & Lund, J.W.G. (1995).  Freshwater Algae: Their Microscopic World Explored.  Biopress, Bristol.

Reynolds, C.S. (1984). The Ecology of Freshwater Phytoplankton. Cambridge University Press, Cambridge.