Natural lenses …

The photograph above is as about as far from Andreas Gursky’s careful constructions, described in the previous post, as it is possible to get.  It is a close-up of a green algal floc Heather noticed whilst on a walk around a local nature reserve.   I guess it fits the general description of “decisive moment” except that it takes a special sort of observer to find any interest at all in such an unprepossessing habitat.

Under the microscope, the floc turned out to be composed of filaments of Spirogyra, with a single helical chloroplast.  Members of this genus (and related genera such as Mougeotia) produce copious mucilage so are always slimy to the touch.  However, this mucilage makes it difficult for the waste gases produced by photosynthesis to diffuse away, leading to the production of bubbles within the mucilage mass.   The interest, today, however, was that these air bubbles are acting as tiny lenses through which it is possible to make out the individual filaments of Spirogyra.

The green floc beside a footpath in Crowtrees local nature reserve from which the other images in this post were derived. 

I should add the caveat here that the photograph was taken with the “super macro” facility of our Olympus TG2 camera but the end-product is, nonetheless, impressive.   It also offers us an insight into the world of the very earliest microscopists.  Anton van Leuwenhoek’s microscopes consisted of a metal plate which held a tiny sphere of glass which acted as a convex-convex lens capable of up to 266x magnification to a resolution of little  more than a micron (1/1000th of a millimetre) (follow this link for more details).  To give an idea of what he might have seen with this, the right hand image below used 400x magnification.

That, however, only tells us part of the story of Anton van Leuwenhoek’s genius.   Whilst we should not underestimate the skill required to make the lenses and their mounts, the other essential element is curiosity.   Curiosity is, itself, multifaceted: in a few weeks we will probably make a trip out to an old quarry where we know we will find several species of orchids, and maybe some excursions to locations new to us but where others have reported interesting assemblages.  That’s one type of curiosity.  However, simply looking harder at the habitats all around us involves a different type of curiosity: a recognition that there is more to know even about things we think we already know about.   The former broadens our experiences, the latter deepens them …

The algal floc at Crowtrees local nature reserve in close-up: left: an extreme macro view of a single bubble from the image at the top of the post and, right: filaments of Spirogyra photographed under the microscope.  Scale bar: 20 micrometres (= 1/50th of a millimetre).

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Do we see through a microscope?

I would have considered this to be a question that barely needs an answer. That, however, was before I read an essay by philosopher Ian Hacking who brings the spirit of logical positivism to the question. When we look through a microscope we see objects that are not “… physical things in a literal sense,”, he writes, “but merely by courtesy of language and pictorial imagination.” Confused? Read on.

First of all, “seeing” is not the same as “looking”.   “Seeing” implies a higher level activity than mere “looking”.   Both involve the reaction of light on the retina, leading to stimulation of the optical nerve.   However, what we see when we look down a microscope differs from what we see when we look at a view out of a window in two important ways. The first is that most of the time our eyes are analysing reflected light, whereas the microscopist mostly (though not always) is looking at transmitted light, light that has passed through the object we are looking at.   The tones we see are a consequence of variations in the proportions of light that are transmitted or absorbed rather than in the proportions that are reflected or absorbed.

The second difference is that a consequence of magnification using an optical microscope is a very shallow depth of field (i.e. the space within which objects are in acceptable focus).   The picture below shows the view down my microscope as I was examining the specimens that I wrote about in my previous post.   In practice, I never actually “saw” this image, and I certainly could not have photographed it, due to the limitations of depth of field. In practice, I was constantly adjusting the focus with my right hand, whilst drawing with my left.   This is a point that Hacking makes: you have to learn to “see” through a microscope. As children we learn to judge distance by correlating visual information with tactile experiences, so microscopists learn to infer three dimensions in their worlds by gentle manipulation of the fine focus mechanism on their microscopes.   “Seeing”, in other words, is far more than just the consequence of light hitting the retina.

Ehen_SC_151216_#1

River Ehen, near the outflow from Ennerdale Water, December 2015. Drawn at x400 magnification.   See images in previous post for indications of scale.

More broadly, we could argue that “seeing” in the microscopic world requires interventions.   The lenses in our microscopes are one type of intervention, of course and without these we would not be able to see tiny objects with clarity.   But this is just the start: we often use stains to adjust the optical properties of certain parts of cells, making it both easier to see these and, in many cases, to infer their composition (iodine binding to starch is a good example).   These stains are often highly toxic so one unwelcome “intervention” is that we necessarily study dead or dying tissues.   The study of diatoms depends upon high resolution mountants, which make the task of viewing structural details of objects that are, essentially, microsocopic lumps of glass mounted between a glass slide and a glass cover slip much easier. This follows a stage in which the cellular contents are dissolved away by the action of strong oxidising agents.   So routine is this stage that many diatomists are in danger of forgetting that the empty glass shell is not really the reality that they seek.

We could argue that microscopic observations approach truth only when they can be validated by an independent means. At low magnifications, the difference between the raw and magnified images are small and individuals can easily validate what they see through a lens.   I know that there are words on a page of writing because I can see them crisply through my reading glasses but I could also, if necessary, hand the page to my son who will be able to confirm that the fuzzy blur that my unaided eyes discern is, indeed, writing.   However, there is a threshold in magnification beyond which this form of validation will not work.   We rely on a network of theoretical “proofs” to confirm the truth in a microscopic image. At one end of the scale this may involve a continuum of consistent observations from the hand lens to medium or high power optical microscope.   At the other end of the scale, it may involve a completely different approach to visualisation (the electron microscope, for example) to corroborate an observation. During my postgraduate days, a fellow student defined the cellular organelles he saw in his electron micrographs as “reproducible artefacts”.   His flippancy, in fact, contains a deep truth: we make our observations on highly manipulated specimens and need to be rigorous in our efforts to confirm their validity.

Hacking’s conclusion was that we see with a microscope, but it is naïve to suggest that we see through a microscope. We may look through a microscope but the images make sense only because of interactions that go beyond patterns of light hitting the retina.   “Seeing”, in other words, is a higher-level process that involves interactions with the brain and with the specimen itself.   Hacking calls on a lot of high power philosophy to make his point; all we need to do is to take care before equating the images we create with the truth.

Happy Christmas.

Wear_Wolsingham_composite

River Wear, Wolsingham: four views down the microscope: top left: January 2009; top right: March 2009; bottom left: June 2009; bottom right: September 2009.  

Reference

Ian Hacking (1981). Do we see through a microscope? Pacific Philosophical Quarterly 62: 305-322.

More about John Carter

John Carter, who I wrote about in my last-but-one post (“Remembering John Carter”) had a great interest in Achnanthes, the old genus to which Platessa bahlsii (see post of 12 March 2014) would have once belonged.   In the early 1960s he wrote a series of papers for the journal The Microscope entitled “The genus Achnanthes as it occurs in British fresh waters”, and which included a key and many of John’s highly detailed drawings (see figure).  “I feel”, he wrote, “that many forms are overlooked simply because of their minute size and the extreme fineness of sculpture on the valve.”   He spent many hours carefully drawing what he could see down his microscope and, indeed, described several new species.  I have long believed that drawing is an important discipline for focussing attention on the properties of organisms and John was an exemplar of this.  Within a few years of his death, however, the advent of high-resolution digital imaging made it much easier to put several individuals from each of several populations alongside one another in order to study this variation more objectively (see the images of Platessa bahlsii as an example).

Planothidium_Carter_1961

An illustration from John Carter’s 1961 paper on the genus Achnanthes.  These drawings show several species most of which would now be placed in the genus Planothidium.

He attempted, along with two other amateur microscopists, to draw all the diatoms then known from Britain, and their Atlas of British Diatoms was published three years after his death in 1996.  It is still sits on the bookshelf beside my microscope, as much for the aesthetic pleasure that I gain from his meticulous drawings of the larger diatoms such as Pinnularia.  The book is now, alas, out of print.

My favourite memory of John Carter? I mentioned to him how I found the German in Hustedt’s 1930 edition of the Susswässerflora von Mitteleuropa much easier to understand than that in Krammer and Lange-Bertalot’s revised version.   He smiled as he recalled a conversation with Lange-Bertalot: “Horst, I said, you speak wonderful English but you write terrible German.”   I recounted this anecdote to a German colleague last year who looked horrified and told me that Lange-Bertalot is admired in Germany for his very meticulous and precise technical German.  This story sums up for me John Carter’s archetypal no-nonsense Yorkshire attitude that treated everyone from students to eminent professors in exactly the same manner.

References

Carter, J.R. (1961).  The genus Achnanthes as it occurs in British fresh waters.  The Microscope 12: 320-325.

Hartley, B., Barber, H.G. & Carter, J.R. (1996).  An Atlas of British Diatoms (edited by P.A. Sims).  Biopress, Bristol.

Remembering John Carter

I wondered, as I re-read the previous post, what my late mentor and friend John Carter would have called the diatom I was writing about.   When I first started looking seriously at diatoms in the early 1990s, there was no-one in my laboratory in Durham with any experience from whom I could learn, and Brian Whitton suggested I went up to visit John at his home near Hawick, in the Scottish Borders.   I rung to arrange a date and, a couple of weeks later, made the two hour drive up through Northumberland and across the border to his house in the small village of Denholm.   Stepping into John’s study was like stepping back in time fifty years: it was dark and dusty, with piles of books and file boxes lining the walls and stacked on tables, along with boxes of microscope slides.  In the fireplace there was some of the equipment that he used to digest his diatom samples – apparatus that really belonged in a laboratory fume cupboard.   And, on a narrow desk against one wall, an old brass microscope equipped with a tilting mirror rather than its own light source.

John_Carter

John Carter in his study at Denholm, in Scotland.  An uncredited photograph from his obituary in Diatom Research

We spent the day in this study, John peering down his microscope and calling out the names of the diatoms he saw along with a commentary on the diatomists he had known (Hustedt, I remember him telling me, was a member of the Nazi party, which made it difficult for him to re-integrate with the scientific community after the war).  I perched on a chair beside him taking notes and occasionally squinting down the microscope to see for myself what he was describing.  I often, too, got a pithy assessment of the state of the slides that I had brought with me.   After a couple of hours of this, we would be summoned by his wife into the dining room for a hearty casserole, and while we ate they would quiz me about my children and talk about their years in the Borders.

Later, back in Durham, I would go back through the slides and try to reconcile my notes with what I could see under the laboratory’s much more modern microscope, ever marvelling at just how much detail John had been able to see with his old equipment.   It was a steep learning curve but, after half a dozen visits to John, it all began to make sense, and I gradually gained the confidence I needed to identify diatoms on my own.   Not long after that we had a telephone call in the laboratory to say that John Carter had died.  I felt that a door onto an older, more civilised, way of doing science had closed.