Love and sex in a tufa-forming stream …

The reason behind my trip to the Lake District a couple of weeks ago was to teach a short course on identification of freshwater macroalgae with Allan Pentecost (see “Heatwave? What heatwave” and subsequent posts for more about last year’s course).   One of the sites we visit with the students is a small stream flowing off Whitbarrow, a Carboniferous limestone outcrop in southern Cumbria.   The bed of the stream is covered with tufa, formed from calcium carbonate precipitated from the water. We bring the students here because there is usually a good variety of cyanobacteria for them to learn to recognise in the field and to sample for later investigation in the laboratory.   Amongst these cyanobacterial growths, however, we also saw a few patches of green filaments on the stream bed, which we also took back with us.

Whitbarrow_tufa_stream_May1

Sampling Whitbarrow tufa stream in May 2015.

These filaments turned out to be growths of the green alga Oedogonium. You may remember that I wrote a post last year with the title “The perplexing case of the celibate alga …” in which I commented that Oedogonium, though a common genus in freshwaters, is difficult to identify to species because this requires the reproductive organs which are rarely seen in the wild.

Our population of Oedogonium, however, was fertile, and this enabled us (Allan, to be strictly honest, as he knows the algae of tufa-forming streams extremely well) to name it.   The images below show the distinctive swollen oogonia within filaments of narrow cells (compare these with the much broader cells observed in “A case of mistaken identity?”). These oogonia look as if they have already fused with the male antheridia to form zygotes, which will eventually be released. These zygotes can lie dormant for a long time, which makes sexual reproduction a useful technique for overcoming adverse conditions (see also: “The River Ehen in March”). Not very romantic, I know, but that’s the reality of life at the unprepossessing end of biodiversity.

Oedogonium_calcareum_Whitba

Oedogonium calcareum from Whitbarrow tufa stream, May 2015, showing oogonium. Arrows indicate position of “caps” (scar tissue from intercalary cell division) a. scale bar: 20 micrometres (= 1/50th of a millimetre); b. & c.: scale bar: 10 micrometres (= 1/100th of a millimetre).

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A record of Achnanthidium trinode from Wales

My ongoing interest in “rare” diatoms (see “The Really Rare Diatom Show” and associated posts) has precipitated some online discussions with Chris Carter about a diatom that he found in one of his recent samples from Tarran y Esgob, a petrifying stream in the Black Mountains (SO 23926 31499). Neither of us had seen it before and, with a little help from Luc Ector, we managed to identify it as Achnanthidium trinode Ralfs in Pritchard 1861 (synonym: Achnanthes trinodis (W. Smith) Grunow in Van Heurck 1880).   Other algae at this site included the Cyanobacteria Nostoc and Rivularia (or Dichothrix) and the diatom Ellerbeckia.

It has a linear outline with a swollen middle portion and capitate ends. Hofmann et al. (2011) describe the length as 15-28 mm and the breadth as 5 – 6 mm. The striae are very fine (30-35 / 10 mm) and barely discernible with the light microscope.   There is a narrow linear axial area on the raphe valve with a small, rounded to lanceolate central area; the rapheless valve is similar but the central area is larger than on the raphe valve.

Achnanthidium_trinode

Achnanthidium trinode from Tarran y Esgob, Wales, January 2015. a & b: raphe valves; c & d: rapheless valves; e & f: girdle views.   Scale bar: 10 micrometres (1/100th of a millimetre).

Hofmann et al. (2011) comment that it is found occasionally in small numbers in oligotrophic, calcareous lakes in Germany and that it was more common in Bavaria in the 1920s before the onset of cultural eutrophication. They regard it as an indicator of very good ecological quality. I had not seen it in the UK before, but it is listed in Hartley’s checklist of diatoms from the British Isles and also in the checklist of Irish diatoms.   A little Googling also revealed a record of the species in The Diatomaceae of the Hull District by Mills and Philip, published in 1901. There is chalk geology in the areas around Hull so this is, perhaps, not surprising given what we know about it’s habitat preferences.   However, I have only two other records on my databases (over 10,000 samples) and in both of these samples it is present in low numbers. This means that it is probably another candidate for our provisional “red list”.   More so because the habitats where Chris’ samples came from (petrifying springs with tufa-forming mosses) are, themselves, endangered.

References

Hartley, B. (1986). A check-list of the freshwater, brackish and marine diatoms of the British Isles and adjoining coastal waters. Journal of the Marine Biological Association of the U.K. 66: 531-610.

Hofmann, G., Werum, M. & Lange-Bertalot, H. (2011). Diatomeen im Süsswassser-Benthos von Mitteleuropa. A.R.G. Gantner Verlag K.G., Rugell.

Mills, F.W. & Philip, R.H. (1901). The Diatomaceae of the Hull District. Transactions of the Hull Scientific and Field Naturalists’ Club 1:157-191, plates 12-28.

More about the habitat in:

Graham, J. & Farr, G. (2014). Petrifying springs in Wales. Field Bryology 112: 19-29.